The product has passed the NMPA(China National Medical Products Administraction) authority certification.

 

Basic Info.

We supply the this Nucleic Acid detection kit( Real-Time Fluorescent RT-PCR), aiming to supply timely and effective detection service to extensive medical organizations. ORF1ab gene and N gene dual-site detection.
Using the human housekeeping gene as an internal standard to effectively prevent false negatives.UNG enzyme + dUTP system to prevent false positives caused by aerosol pollution.

[Product Name] Nucleic Acid detection kit( Real-Time Fluorescent RT-PCR)



For in vitro diagnostic use only. For professional use only


Advantages and features:

1. Multiple fluorescence quantitative PCR, single tube reaction reduces the operation steps and improves the detection flux;

2. Premixed reaction liquid, more convenient operation;

3. Single tube preloading reagent to meet more laboratory requirements;

4. Rapid amplification, 40-75 minutes to complete the amplification reaction;

Laboratory tests showed that the amplification time of ABI7500 / slan-96 / LightCycler480 was < 75 min, and that of kinnomix C7 was < 40 min





Performance verification:
The noval Nucleic Acid detection kit(Real-Time Fluorescent RT-PCR) developed by bio-co., LTD., has passed the test of the Chinese center for disease control and prevention of viral diseases on February 1. According to the technical guidelines for laboratory testing of novel infected pneumonia (3rd edition), it is concluded that the fluorescence detection results of novel  virus detection kit with two different targets are in good consistency with the laboratory detection methods. Moreover, hundreds of cases of clinical data were verified in many first-class hospitals in wuhan.


 

[Precautions]
1. Please read the entire Instructions carefully before using the kit.
2. This product does not contain nucleic acid extraction reagent, which needs to be prepared by the user.
Please refer to [Main Components] for details.
3. Please strictly follow the management practices of genetic amplification testing laboratories as issued by the industrial administrative department in charge.
4. This kit is an in vitro detection reagent, and the operating personnel must be professionally trained.
5. The entire detection process shall be strictly conducted in three areas, i.e.,Reagent Preparation Area, Specimen Preparation Area and Amplification Area.The instruments, equipment, consumables and work clothes used in each area shall be independent and dedicated.The workbench shall be cleaned immediately after the experiment and be disinfected.
6. Please use disposable dedicated centrifuge tubes, calibrated pipettes, tips with filter element, as well as disposable gloves that do not contain fluorescent materials. Gloves must be replaced frequently.
7. The components in the kit shall be used within the validity period. Do not mix reagents of different batches.
8. All reagents must be thawed completely, mixed well and centrifuged at 6,000rpm for a few seconds before use.
9. Instruments and equipment, such as operating tables, pipettes,centrifuges and PCR analyzer, shall be disinfected frequently with 10% sodium hypochlorite or 75% alcohol, UV or ozone.
10. Try to avoid air bubbles in the reaction tube. The tube cap must be tightened.
11. After the amplification is completed, take out the reaction tube, seal it in a special plastic bag, and discard it at the designated place.
12. The test specimens involved with this kit are considered to be infectious substances, and any operation and processing must meet the relevant requirements of General Biosafety Standard for Microbiological and
Biomedical Laboratories and Medical Waste Management Regulations released by the Ministry of Health of the P.R.C.
13. Test kit for professional use only