Product Description
Product Name:Usual Name: Virus Nucleic Acid Extraction Kit (Adsorption Column Method).
Packaging Specifications:50Testing/box.
Expected Usage:This kit is used to extract virus DNA/RNA from plasma, serum, ascites, cell culture supernatant,
cerebrospinal.fluid, stool, tissue and other samples. The extracted nucleic acid can be used for clinical molecular biology detection.
Product Principle:This kit uses a unique buffer system and a centrifugal adsorption column that specifically binds virus DNA/RNA.
Centrifugal adsorption columns can efficiently and specifically adsorb viral DNA/RNA, and remove impurities and proteins as much
as possible.
Packaging Specifications:50Testing/box.
Expected Usage:This kit is used to extract virus DNA/RNA from plasma, serum, ascites, cell culture supernatant,
cerebrospinal.fluid, stool, tissue and other samples. The extracted nucleic acid can be used for clinical molecular biology detection.
Product Principle:This kit uses a unique buffer system and a centrifugal adsorption column that specifically binds virus DNA/RNA.
Centrifugal adsorption columns can efficiently and specifically adsorb viral DNA/RNA, and remove impurities and proteins as much
as possible.
Instructions
1.Take 200μlof plasma, serum,ascites, cell culture supernatant, cerebrospinal fluid, tissue milled
supernatant and other liquids (do not use whole blood), place in a clean 1.5mL centrifuge tube, add 200μl
Lysis Solution to the centrifuge tube,vortex for 10s or mix by inversion 10 times.
2. Let stand for 10 minutes at room temperature, then add 200μl of Absolute Ethanol to the centrifuge tube,
and mix by inversion for 15s.
3. Put the Adsorption Column into the Collection Tube, transfer all the above liquid into the Adsorption
Column, and centrifuge for 1 minute using a palm centrifuge, and discard the filtrate.
4. Add 600μlof Washing BufferI to the Adsorption Column (confirm that absolute ethanol was added to
Washing Solution I before use), and centrifuge for 1 minute using a palm centrifuge, and discard the filtrate.
5. Add 600μlof Washing Buffer to the Adsorption Column, centrifuge for 1 minute using a palm centrifuge,
and discard the filtrate.
6. Return the Adsorption Column to the collection tube and centrifuge for 2 minutes using a palm centrifuge,
discard the filtrate and the collection tube.
7. Place the Adsorption Column in a RNase/DNase-free 1.5ml centrifuge tube, add 50μlElution Solution in the
center of the A dsorption Column membrane, and leave it at room temperature for 1 minute. Centrifuge for
1 minute with a palm centrifuge, discard the Adsorption Column. The nucleic acid collected in the 1.5 ml
centrifuge tube was stored at -20ºC.
supernatant and other liquids (do not use whole blood), place in a clean 1.5mL centrifuge tube, add 200μl
Lysis Solution to the centrifuge tube,vortex for 10s or mix by inversion 10 times.
2. Let stand for 10 minutes at room temperature, then add 200μl of Absolute Ethanol to the centrifuge tube,
and mix by inversion for 15s.
3. Put the Adsorption Column into the Collection Tube, transfer all the above liquid into the Adsorption
Column, and centrifuge for 1 minute using a palm centrifuge, and discard the filtrate.
4. Add 600μlof Washing BufferI to the Adsorption Column (confirm that absolute ethanol was added to
Washing Solution I before use), and centrifuge for 1 minute using a palm centrifuge, and discard the filtrate.
5. Add 600μlof Washing Buffer to the Adsorption Column, centrifuge for 1 minute using a palm centrifuge,
and discard the filtrate.
6. Return the Adsorption Column to the collection tube and centrifuge for 2 minutes using a palm centrifuge,
discard the filtrate and the collection tube.
7. Place the Adsorption Column in a RNase/DNase-free 1.5ml centrifuge tube, add 50μlElution Solution in the
center of the A dsorption Column membrane, and leave it at room temperature for 1 minute. Centrifuge for
1 minute with a palm centrifuge, discard the Adsorption Column. The nucleic acid collected in the 1.5 ml
centrifuge tube was stored at -20ºC.
FAQ
1. Could you provide free samples for our testing before we place the order?
2. Do you support OEM/ODM order?
Re: Sure, we'd like to offer free samples for you, but the shipping cost should be paid by buyers.
2. Do you support OEM/ODM order?
Re: Of couse, we support OEM/ODM order.
3. Do you have any certifications?
Re: We have CFDA, CE, ISO, SGS etc. certifications.
4. What is your best delivery time?
Re: It will depend on your order quantities. We will confirm with you after you placed the order.
5. I'm a distributor/small wholesaler, could you accept small order?
Re:Yes,we could.